The Klondike Gold Rush National Historical Park curatorial center is one of the largest repositories of Klondike Gold Rush material with over 250,000 items in storage. The collection consists of material from over 50 excavations in the historic district, the nearby historic town of Dyea, and the Chilkoot and White Pass Trails. The historic district excavations were from beneath and around buildings prior to restoration. The various buildings excavated were two saloons, a liquor store, railroad depot, bakery, cigar and confectionery store, dry goods store, tailor and furrier shop, paint and wallpaper store, transportation company, catholic church rectory, mission house, the home of the founder of Skagway, and a residential block outside the historic district. Many of these collections have been analyzed and reports are in progress or have been completed.

The Park collection also consists of over 1,000 mounted herbarium specimens which are a valuable research tool to aid in the understanding of this unique Pacific Coastal Rain Forest/Alpine Tundra ecosystem.